Using Solid-Phase Microextraction Coupled with Reactive Carbon Fiber Ionization-Mass Spectrometry for the Detection of Aflatoxin B1 from Complex Samples

Aflatoxin B1 (AFB1) is a common mycotoxin present in agricultural and food products. Therefore, rapid screening methods must be developed for AFB1 detection with high sensitivity good selectivity. In this study, we an analytical method based on the combination of solid-phase microextraction (SPME) carbon fiber ionization (CFI)-mass spectrometry (MS) to detect presence trace from complex samples. A pencil lead (type 2B, length: ~2.5 cm) sharp end (diameter: ~150 ?m) was used as SPME emitter CFI-MS analysis. Owing graphite structure lead, can trapped through ?–? interactions. After adsorbing AFB1, directly introduced pipette tip (length: ~0.7 cm; inner diameter: ~0.6 mm), placed close (~1 mm) inlet mass spectrometer, applied voltage (?4.5 kV) situ elution direct electric contact SPME-CFI setup not required. Followed by introduction solvent (10 ?L) (acetonitrile/ethanol/deionized water, 2:2:1 (v/v/v)) tip, electrospray generated containing reactive SPME-CFI-MS strategy employed further identify improve capacity using our approach. Butylamine added solvent, which then inserted fiber. Butylamine-derivatized readily appeared resultant spectrum. The lowest detectable concentration against approach ~1.25 nM. Our distinguish AFG1 mixture peanut extract